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shrna plasmid with pspax2  (Addgene inc)


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    Structured Review

    Addgene inc shrna plasmid with pspax2
    Shrna Plasmid With Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pspax2/psPAX2+(Plasmid+%2312260)/pmc13050104-51-9-13
    Average 98 stars, based on 15527 article reviews
    shrna plasmid with pspax2 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Infection:

    Article Title: HER2 heterogeneous breast cancer models reveal novel therapeutic targets and subclonal dynamics during evolution to resistance to HER2-targeted therapies
    Article Snippet: .. 921 922 Viral Infection and constructs 923 For virus production, HEK293FT cells (RRID:CVCL_6911) were transfected using Lipofectamine 924 3000 (Thermo Fisher Scientific) with lentiviral packaging plasmids pMD2.G 925 (RRID:Addgene_12259) and psPAX2 (RRID:Addgene_12260), and indicated plasmids. .. The viral 926 supernatant was collected 48h hours later, filtered through a 0.45 μm syringe filter and added to 927 cells with 8 μg/mL Polybrene (Millipore).

    Construct:

    Article Title: HER2 heterogeneous breast cancer models reveal novel therapeutic targets and subclonal dynamics during evolution to resistance to HER2-targeted therapies
    Article Snippet: .. 921 922 Viral Infection and constructs 923 For virus production, HEK293FT cells (RRID:CVCL_6911) were transfected using Lipofectamine 924 3000 (Thermo Fisher Scientific) with lentiviral packaging plasmids pMD2.G 925 (RRID:Addgene_12259) and psPAX2 (RRID:Addgene_12260), and indicated plasmids. .. The viral 926 supernatant was collected 48h hours later, filtered through a 0.45 μm syringe filter and added to 927 cells with 8 μg/mL Polybrene (Millipore).

    Virus:

    Article Title: HER2 heterogeneous breast cancer models reveal novel therapeutic targets and subclonal dynamics during evolution to resistance to HER2-targeted therapies
    Article Snippet: .. 921 922 Viral Infection and constructs 923 For virus production, HEK293FT cells (RRID:CVCL_6911) were transfected using Lipofectamine 924 3000 (Thermo Fisher Scientific) with lentiviral packaging plasmids pMD2.G 925 (RRID:Addgene_12259) and psPAX2 (RRID:Addgene_12260), and indicated plasmids. .. The viral 926 supernatant was collected 48h hours later, filtered through a 0.45 μm syringe filter and added to 927 cells with 8 μg/mL Polybrene (Millipore).

    Article Title: ATF3-dependent formation of inclusion bodies in polyQ-expressing human iPSC-derived neurons confers cellular protection.
    Article Snippet: .. For standard virus generation, 50–70% cell confluence of HEK293 cells were transfected with lentiviral packaging vectors; pMD2.G (Addgene #12259), psPAX2 (Addgene #12260), vector of interest in a ratio of 1:3:4, respectively, following the Mirus reagent (transIT-LT1) protocol. ..

    Transfection:

    Article Title: HER2 heterogeneous breast cancer models reveal novel therapeutic targets and subclonal dynamics during evolution to resistance to HER2-targeted therapies
    Article Snippet: .. 921 922 Viral Infection and constructs 923 For virus production, HEK293FT cells (RRID:CVCL_6911) were transfected using Lipofectamine 924 3000 (Thermo Fisher Scientific) with lentiviral packaging plasmids pMD2.G 925 (RRID:Addgene_12259) and psPAX2 (RRID:Addgene_12260), and indicated plasmids. .. The viral 926 supernatant was collected 48h hours later, filtered through a 0.45 μm syringe filter and added to 927 cells with 8 μg/mL Polybrene (Millipore).

    Article Title: ATF3-dependent formation of inclusion bodies in polyQ-expressing human iPSC-derived neurons confers cellular protection.
    Article Snippet: .. For standard virus generation, 50–70% cell confluence of HEK293 cells were transfected with lentiviral packaging vectors; pMD2.G (Addgene #12259), psPAX2 (Addgene #12260), vector of interest in a ratio of 1:3:4, respectively, following the Mirus reagent (transIT-LT1) protocol. ..

    Plasmid Preparation:

    Article Title: New selective and allosteric FLT3 inhibitors show efficacy against resistant acute myeloid leukemia cells
    Article Snippet: .. psPAX2 , Addgene , Plasmid #12260. .. pMD2.G , Addgene , Plasmid #12259.

    other:

    Article Title: SplitTurboID mapping of dimeric protein phosphatase complex interactomes
    Article Snippet: psPAX2 , Addgene , Cat#12260 RRID:Addgene_12260.

    shRNA:

    Article Title: Hemidesmosomes and Notch signaling regulate epidermal differentiation via delamination.
    Article Snippet: .. Both versions of the shRNA were packaged into lentivirus by using HEK 293FT cells, pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) helper plasmids. ..

    Sequencing:

    Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways
    Article Snippet: .. After confirmation of the inserted sequence, the lentiCRISPRv2-puro vectors were co-transfected along with the psPAX2 (cat. no. 12260; Addgene, Inc.) and pCMV–VSV-G (Addgene_8454; Addgene, Inc.) vectors into Lenti-X 293T cells (CVCL_4401; Takara Bio) using FuGENE HD (Promega Corporation) to generate infectious lentivirus. ..



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